Catalog Number - 68040-25P

Cogent NP Amino is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity silica with a propyl amino bonded phase, it provides excellent selectivity for carbohydrates, sugars, organic acids, and other polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations.

68040-25P is an analytical HPLC column packed with 10 µm NP Amino particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm length maximizes resolving power and peak capacity, making it ideal for complex separations requiring the highest chromatographic performance. The 10 µm particle size supports higher flow rates and lower operating backpressure compared with smaller particles. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on analyte properties, mobile phase composition, and instrument configuration.

This column is particularly effective for sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. As with all amino phases, this column is suitable for both Normal Phase and HILIC applications when enhanced retention of polar analytes is required.

Key Benefits

• NP Amino stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded phase

• High-purity silica support

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Enhanced retention of polar compounds

• Excellent for sugars and carbohydrates

• Low operating backpressure with 10 µm particles

• Maximum resolving power for complex separations

Typical Applications

• USP L8 methods

• HILIC separations

• Normal Phase chromatography

• Sugar analysis

• Carbohydrate analysis

• Organic acid analysis

• Carboxylic acid analysis

• Food and beverage testing

• Pharmaceutical analysis

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: NP Amino

• USP Classification: L8

• Chromatography Mode: Normal Phase / HILIC

• Bonded Phase: Propyl Amino

• Endcapping: Not Endcapped

• Particle Size: 10 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Packed and shipped in Normal Phase solvents

• Suitable for both Normal Phase and HILIC chromatographic modes

• Excellent retention of sugars, carbohydrates, and organic acids

• Extended column length maximizes resolution and peak capacity

• Frequently used for method development and preparative chromatography scouting

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